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The theoretical molar ellipticity at 222 nm (assuming 100% helicity) is then , The experimental values (and hence α-helix fractions f a ) are as follows: For a fresh sample For a 40-day aged sample Fluorescence Spectroscopy Fluoroscence of tryptophan in semaglutide was excited at λ exc = 295 nm, and fluorescence emission spectra were measured using a Cary Eclipse spectrofluorometer (Agilent, Didcot, UK) with excitation and emission slits of 5 nm
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